9NLG
CBASS Pseudomonas syringae Cap5 tetramer with 3'2'-c-GAMP cyclic dinucleotide ligand (His56Ala mutant without Mg2+ ions)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-14 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.564, 77.833, 80.989 |
| Unit cell angles | 63.40, 80.84, 79.17 |
Refinement procedure
| Resolution | 45.870 - 1.640 |
| R-factor | 0.1572 |
| Rwork | 0.156 |
| R-free | 0.18060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.773 |
| Data reduction software | autoPROC (BUILT 20220820) |
| Data scaling software | autoPROC (BUILT 20220820) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.054 | 1.683 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.055 | 0.547 |
| Number of reflections | 173836 | 8697 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 94.7 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.998 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 10 mM Trisodiumcitrate pH 9.0, 28% PEG6000 |
