9NL1

Crystal structure of Fab MAM01 in complex with junctional region peptide from circumsporozoite protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-18
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	45.418, 72.805, 136.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.430 - 2.710
R-factor	0.2604
Rwork	0.255
R-free	0.31270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.545
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.710	2.710
Rmerge	0.105	0.449
Rpim	0.054
Number of reflections	13394	641
<I/σ(I)>	25.15
Completeness [%]	92.8	92.9
Redundancy	5.2
CC(1/2)	0.991	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.085 M Tris pH 8.5, 0.17 M sodium acetate, 15% (v/v) glycerol and 25.5 % (w/v) polyethylene glycol 4000