9NK0

Prenylated-FMN maturase PhdC from Mycolicibacterium fortuitum (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	I 2 2 2
Unit cell lengths	60.083, 76.697, 80.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.348 - 1.520
Rwork	0.127
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.977
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.350	1.550
High resolution limit [Å]	1.520	1.520
Rmerge	0.025	0.980
Rmeas	0.027	0.106
Rpim		0.570
Number of reflections	28916	9655
<I/σ(I)>	46	16.9
Completeness [%]	99.7	99.8
Redundancy	6.6	6.7
CC(1/2)	1.000	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	2:1 protein (20mM bistris pH 7.2, 200mM KCl, 1mM MnCl2, 5% glycerol) to condition (30% w/v PEG 5000 MME, 100 mM MES/ Sodium hydroxide pH 6.5, 200 mM ammonium sulfate)