Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 145.390, 145.390, 145.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.400 - 2.530 |
| R-factor | 0.2326 |
| Rwork | 0.230 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.811 |
| Data reduction software | XDS (BUILT 20241002) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.806 | 2.578 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.197 | 1.891 |
| Rmeas | 0.211 | 2.021 |
| Number of reflections | 16285 | 861 |
| <I/σ(I)> | 7.4 | 1.4 |
| Completeness [%] | 95.1 | |
| Redundancy | 8 | |
| CC(1/2) | 0.993 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 mM MES, 1.0 M sodium citrate, 5 mM TCEP |
