9NIP
Mycobacterium tuberculosis Small RNA-Binding protein (SRB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.980, 87.994, 122.488 |
| Unit cell angles | 90.02, 90.03, 90.03 |
Refinement procedure
| Resolution | 41.400 - 2.620 |
| R-factor | 0.2783 |
| Rwork | 0.275 |
| R-free | 0.33510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS (BUILT 20241002) |
| Data scaling software | Aimless (0.8.2) |
| Phasing software | MoRDa (1.4.08) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 2.660 |
| High resolution limit [Å] | 2.615 | 2.615 |
| Rmerge | 0.228 | 1.629 |
| Rmeas | 0.247 | 1.806 |
| Rpim | 0.096 | 0.762 |
| Number of reflections | 110352 | 3522 |
| <I/σ(I)> | 4.9 | 1.1 |
| Completeness [%] | 99.7 | 96.6 |
| Redundancy | 6.3 | 5.1 |
| CC(1/2) | 0.993 | 0.366 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M sodium acetate trihydrate pH 4.5, 3.0 M sodium chloride |
