9NFL
Tuna P-glycoprotein Apo Conformation 4
EM specimen
| Vitrification | Yes |
| Instrument | SPT LABTECH CHAMELEON |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.02 |
| Number of Particle | 4800000 |
| Software | cryoSPARC,PHENIX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
