9NCY
Fab1392 in complex with the C-terminal alpha-TSR domain of the P. falciparum circumsporozoite protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.355, 150.146, 136.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.150 - 1.630 |
| R-factor | 0.1753 |
| Rwork | 0.174 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.085 | 1.270 |
| Rmeas | 0.088 | 1.404 |
| Rpim | 0.024 | 0.582 |
| Number of reflections | 114968 | 3943 |
| <I/σ(I)> | 24.5 | 1 |
| Completeness [%] | 94.5 | 65.7 |
| Redundancy | 12.3 | 4.9 |
| CC(1/2) | 0.999 | 0.437 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG-600, 10% (v/v) glycerol, 0.5 M NH4-sulfate, 0.1 M Tris pH 7.0, final pH 5.6. |
