9NCI

[5,7,7,P-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (5,7,7) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	106.158, 106.158, 90.212
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.430 - 4.710
R-factor	0.2446
Rwork	0.242
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.738
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.390	64.390	5.483
High resolution limit [Å]	4.708	10.239	4.708
Number of reflections	1368	195	197
<I/σ(I)>	8.6
Completeness [%]	83.0
Redundancy	7.9
CC(1/2)	0.999	0.999	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate