9NC4

[2,8,9-2] Shifted tensegrity triangle with an (arm,center,arm) distribution of (2,8,9) base pairs and 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	H 3
Unit cell lengths	111.893, 111.893, 72.681
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.030 - 4.290
R-factor	0.2909
Rwork	0.289
R-free	0.31720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.947	55.946	4.851
High resolution limit [Å]	4.290	9.867	4.292
Number of reflections	1514	188	191
<I/σ(I)>	9.3
Completeness [%]	83.7
Redundancy	10.7
CC(1/2)	0.998	0.997	0.246

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate