9N9X

Structure of HPK1 with C5 bound at its active site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	91
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	45.120, 75.870, 86.060
Unit cell angles	90.00, 99.48, 90.00

Refinement procedure

Resolution	35.000 - 2.300
R-factor	0.22727
Rwork	0.225
R-free	0.27615
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.925
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.131	0.639
Rmeas	0.158	0.758
Rpim	0.087	0.405
Total number of observations	85274	8579
Number of reflections	25468	2508
<I/σ(I)>	8.9	2.1
Completeness [%]	99.6
Redundancy	3.3	3.4
CC(1/2)	0.973	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	not provided by author