9N7Z
Crystal structure of CT-SLiM-deleted human Drp1 GTPase domain-BSE fusion protein in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.743, 150.611, 87.607 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 32.880 - 2.510 |
| R-factor | 0.2457 |
| Rwork | 0.243 |
| R-free | 0.29030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DIALS (3.18.0-gbecf27540-release) |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_5533) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.730 | 2.580 |
| High resolution limit [Å] | 2.510 | 2.540 |
| Rmerge | 0.624 | 3.739 |
| Rmeas | 0.735 | 4.399 |
| Rpim | 0.386 | 2.301 |
| Total number of observations | 8693 | |
| Number of reflections | 48563 | 2414 |
| <I/σ(I)> | 1.8 | 0.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.923 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.1 M Sodium citrate, pH 5.0, 28 % PEG 3000, 6 mg/ml protein, 1:1 ratio |
