9N5P

CRYSTAL STRUCTURE OF HUMAN IGG2 FC FRAGMENT-FC-GAMMA RECEPTOR IIA COMPLEX H131 VARIANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	161.853, 66.949, 71.179
Unit cell angles	90.00, 94.18, 90.00

Refinement procedure

Resolution	27.550 - 2.010
R-factor	0.1971
Rwork	0.195
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.848
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.135	0.493
Rpim	0.078	0.339
Number of reflections	44826	1928
<I/σ(I)>	28.7	1.8
Completeness [%]	89.5	77.2
Redundancy	3.6	2.6
CC(1/2)	0.986	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.1 M HEPES pH 7.5, 25% PEG 2000 MME