9N5G
RNA polymerase II elongation complex with 8-oxoG at +1 site, ATP in both A- and E-site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.784, 222.488, 192.287 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 48.060 - 3.150 |
| R-factor | 0.2209 |
| Rwork | 0.220 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.632 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.060 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.378 | 3.489 |
| Rmeas | 0.405 | 3.740 |
| Rpim | 0.146 | 1.344 |
| Total number of observations | 879890 | 43765 |
| Number of reflections | 114545 | 5655 |
| <I/σ(I)> | 5.4 | 0.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | 7.7 |
| CC(1/2) | 0.989 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
