9N5E
RNA polymerase II elongation complex with 8-oxoG at +1 site, AMPCPP in E-site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.718, 222.926, 193.285 |
| Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
| Resolution | 48.960 - 3.750 |
| R-factor | 0.2498 |
| Rwork | 0.248 |
| R-free | 0.29120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 3.670 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.574 | 4.804 |
| Rmeas | 0.616 | 5.154 |
| Rpim | 0.222 | 1.862 |
| Total number of observations | 608331 | 34279 |
| Number of reflections | 79511 | 4502 |
| <I/σ(I)> | 4.1 | 0.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 | 7.6 |
| CC(1/2) | 0.984 | 0.355 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
