9N5C

RNA polymerase II elongation complex with 8-oxoG at +1 site, CMPCPP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.984
Spacegroup name	C 1 2 1
Unit cell lengths	162.638, 222.985, 192.256
Unit cell angles	90.00, 98.55, 90.00

Refinement procedure

Resolution	48.770 - 3.600
R-factor	0.2533
Rwork	0.252
R-free	0.29730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.699
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	3.600
High resolution limit [Å]	3.530	3.530
Rmerge	0.514	5.842
Rmeas	0.551	6.262
Rpim	0.199	2.249
Total number of observations	636204	34852
Number of reflections	82990	4521
<I/σ(I)>	5.2	0.5
Completeness [%]	99.9
Redundancy	7.7	7.7
CC(1/2)	0.985	0.234

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000