9N5B
RNA polymerase II elongation complex containing 8-oxoG at +1 site, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.839, 221.722, 190.842 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 46.280 - 3.100 |
| R-factor | 0.2138 |
| Rwork | 0.213 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.694 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.830 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.410 | 3.831 |
| Rmeas | 0.439 | 4.105 |
| Rpim | 0.158 | 1.471 |
| Total number of observations | 998460 | 49801 |
| Number of reflections | 129746 | 6439 |
| <I/σ(I)> | 6.3 | 0.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | 7.7 |
| CC(1/2) | 0.983 | 0.228 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
