9N4B

RhoA GTPase R70A bound to GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-08-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	59.988, 59.988, 150.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.410 - 3.000
R-factor	0.2042
Rwork	0.199
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.161
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.410	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.054	0.236
Rmeas	0.077
Number of reflections	13211	1150
<I/σ(I)>	10.82	2.41
Completeness [%]	94.4	86.61
Redundancy	1.8	1.7
CC(1/2)	0.998	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2 uL protein (8 mg/mL RhoA R70A bound to GTPgammaS in 25 mM Tris-HCl pH8.0, 2 mM MgCl2, and 10 mM BME) + 2 uL crystallization reagent sitting above 500 uL crystallization reagent. Crystallization reagent: 250 mM ammonium sulfate, 1.50 M lithium sulfate, and 100 mM sodium citrate pH 5.6