9N4A

Wild-type RhoA GTPase bound to GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	119.100, 60.778, 70.031
Unit cell angles	90.00, 123.71, 90.00

Refinement procedure

Resolution	45.886 - 1.947
R-factor	0.1808
Rwork	0.178
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.219
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.886	2.020
High resolution limit [Å]	1.947	1.950
Rmerge	0.042
Rmeas	0.059	0.514
Rpim	0.046	0.276
Number of reflections	30326	2661
<I/σ(I)>	12.2	2.47
Completeness [%]	98.5	83.43
Redundancy	3.6	3.3
CC(1/2)	0.995	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.18	2 uL protein (10 mg/mL RhoA-GppNHp, 25 mM Tris-HCl pH 8.0, 2 mM MgCl2, 10 mM BME) + 2 uL of crystallization solution over 500 uL reservoir of crystallization solution. Crystallization solution: 25% dioxane, 17% PEG 8000, and 100 mM HEPES pH 6.9