9N3A

N-Terminal Domain of CRISPR-associated DinG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2024-06-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	I 1 2 1
Unit cell lengths	27.669, 39.304, 83.097
Unit cell angles	90.00, 95.05, 90.00

Refinement procedure

Resolution	26.870 - 1.550
R-factor	0.1334
Rwork	0.132
R-free	0.16170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	DIALS (2021.11.1)
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2-5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.870	35.510	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.060	0.037	0.230
Rmeas	0.073	0.045	0.326
Rpim	0.041	0.026	0.230
Number of reflections	18483	2123	34
<I/σ(I)>	21.1	45.8	1.3
Completeness [%]	73.3	99.57	5.45
Redundancy	2.9	3.08	1.09
CC(1/2)	0.997	0.996	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	Mother liquor consisted of 18% PEG 3350, 0.1M Na citrate buffer (pH 4.0), and 0.2M Ammonium Sulfate. Mother liquor was mixed with concentrated protein (12 mg/ml) at a ratio of 2:1.5 (protein solution : ML)