9N32
PilU from Pseudomonas aeruginosa: C3ocococ conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.999614 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 191.035, 191.035, 191.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 4.540 |
| R-factor | 0.2826 |
| Rwork | 0.282 |
| R-free | 0.29860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.379 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.700 |
| High resolution limit [Å] | 4.540 | 4.540 |
| Rpim | 0.062 | 0.440 |
| Number of reflections | 25631 | 2584 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.0 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 150 mM sodium chloride, 250 mM ammonium sulfate, 50 mM HEPES, pH 7.0, 40 mM citric acid, 20% glycerol, 23% PEG8000, 100 mM Tris, pH 9.5, 0.8 M lithium chloride |
