9N2T

Structure of GDP-bound GM4951_D125G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	297.816, 58.909, 100.679
Unit cell angles	90.00, 92.03, 90.00

Refinement procedure

Resolution	50.010 - 2.600
R-factor	0.23426
Rwork	0.233
R-free	0.26689
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	51392	1867
<I/σ(I)>	16.56
Completeness [%]	95.1
Redundancy	4.2
CC(1/2)	0.990	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	35% tacsimate pH 7.0