9N2S
Structure of GDP-bound GM4951_N86K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.552, 67.360, 70.927 |
| Unit cell angles | 106.58, 100.73, 106.78 |
Refinement procedure
| Resolution | 47.910 - 3.310 |
| R-factor | 0.27721 |
| Rwork | 0.275 |
| R-free | 0.32684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 11878 | 534 |
| <I/σ(I)> | 13.77 | |
| Completeness [%] | 89.9 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.966 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.5 and 15% PEG3350 |
