9N2R
Structure of GTP-bound GM4951
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.759, 100.245, 152.347 |
| Unit cell angles | 92.31, 101.24, 89.86 |
Refinement procedure
| Resolution | 46.020 - 2.750 |
| R-factor | 0.21062 |
| Rwork | 0.209 |
| R-free | 0.24659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 2.800 |
| High resolution limit [Å] | 2.754 | 2.754 |
| Number of reflections | 78604 | 2293 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 88.1 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.979 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1M succinic acid pH 7.0, 0.1 M HEPES pH 7.0, and 1% (wt/vol) PEG-MME 2000 |
