9N2Q

Structure of GDP-bound GM4951

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	60.323, 100.640, 152.355
Unit cell angles	87.49, 78.52, 89.91

Refinement procedure

Resolution	49.720 - 2.510
R-factor	0.23195
Rwork	0.230
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.381
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	2.540
High resolution limit [Å]	2.508	2.508
Number of reflections	108885	4137
<I/σ(I)>	8.7
Completeness [%]	91.1
Redundancy	2.5
CC(1/2)	0.754	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	36% tacsimate pH 7.0