9N0O

CryoEM structure of Rv2531c dimer from Mycobacterium tuberculosis

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	JEOL CRYO ARM 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Phase Plate	OTHER
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.8
Software	cryoSPARC,SerialEM,PHENIX
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION