9MYT

EatA-EatI complex from Mycobacterium abscessus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.96863
Spacegroup name	P 1 21 1
Unit cell lengths	51.584, 99.521, 92.092
Unit cell angles	90.00, 102.48, 90.00

Refinement procedure

Resolution	89.920 - 1.700
R-factor	0.1617
Rwork	0.160
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.848
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.920	1.732
High resolution limit [Å]	1.700	1.700
Number of reflections	89875	1394
<I/σ(I)>	6.3
Completeness [%]	90.6
Redundancy	6.4
CC(1/2)	0.989	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.075 M Magnesium chloride hexahydrate, 0.1 M Sodium cacodylate pH 6.5, 30 % w/v PEG 2000 MME