9MYM

Fertilization IZUMO1 Protein Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	I 4 2 2
Unit cell lengths	103.454, 103.454, 139.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.577 - 1.843
R-factor	0.1706
Rwork	0.170
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.634
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.580	1.900
High resolution limit [Å]	1.840	1.840
Number of reflections	32920	2921
<I/σ(I)>	27.5
Completeness [%]	100.0
Redundancy	20
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	200 mM CaCl2, 100 nM HEPES pH 7.5, 28% PPG P400