9MXI
Crystal Structure of synthetic antibody, COP-2, in complex with the C-terminal domain of Clostridium perfringens Enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 110.929, 197.434, 115.056 |
| Unit cell angles | 90.00, 109.54, 90.00 |
Refinement procedure
| Resolution | 29.662 - 2.200 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.712 |
| Data reduction software | DIALS (0.82) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.092 | 1.449 |
| Rmeas | 0.131 | |
| Rpim | 0.092 | |
| Number of reflections | 116945 | 23320 |
| <I/σ(I)> | 5.15 | 0.85 |
| Completeness [%] | 96.8 | 94.8 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.989 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277.15 | 200 mM sodium chloride, 1.75 M ammonium sulfate, and 100 mM sodium cacodylate pH 6.6 |
