9MVR

AI-designed Cas13 anti-CRISPR AIcrVIA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95365
Spacegroup name	P 21 21 21
Unit cell lengths	33.403, 62.900, 84.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 1.940
R-factor	0.2038
Rwork	0.199
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.460	1.970
High resolution limit [Å]	1.940	1.940
Rmerge	0.087	1.050
Rpim	0.036	0.430
Number of reflections	25959	1300
<I/σ(I)>	10.5	1.08
Completeness [%]	99.4	90.36
Redundancy	6.8	6.5
CC(1/2)	0.990	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	298	0.1M Bis-Tris propane, 2.6M ammonium sulfate