9MV0
Structure of HKU5 spike C-terminal domain in complex with ACE2 from Pipistrellus abramus
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON APOLLO (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.2 |
| Number of Particle | 2125866 |
| Software | crYOLO,cryoSPARC,Topaz,PHENIX,ISOLDE,SerialEM,UCSF ChimeraX,Coot |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
