9MTN
Crystal structure of KwaB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 |
| Unit cell lengths | 283.105, 283.105, 76.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.280 - 3.920 |
| R-factor | 0.2417 |
| Rwork | 0.238 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Data reduction software | AUTOMAR |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.280 | 4.090 |
| High resolution limit [Å] | 3.880 | 3.880 |
| Number of reflections | 20404 | 2586 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.4 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.972 | 0.303 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 290 | 0.1 M Tris, pH 8.5, 5% PEG8000 |
