9MSK

Structure of hepatitis C virus envelope glycoprotein E2 core from isolate H77 bound to neutralizing antibody RM3-26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	79.541, 73.949, 122.451
Unit cell angles	90.00, 90.44, 90.00

Refinement procedure

Resolution	36.970 - 2.230
R-factor	0.2639
Rwork	0.263
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.609
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.970	2.310
High resolution limit [Å]	2.230	2.230
Number of reflections	33892	1465
<I/σ(I)>	7.7
Completeness [%]	98.5	85.7
Redundancy	6.1
CC(1/2)	0.990	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium thiocyanate, 20% w/v PEG3350