9MSA
Alpha-ketoisovalerate decarboxylase (Kivd) from Synechocystis sp. PCC 6803 with substitution S286T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.465, 81.731, 124.981 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.790 |
| R-factor | 0.19156 |
| Rwork | 0.189 |
| R-free | 0.23391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.479 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.810 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.110 | 0.026 |
| Rmeas | 0.120 | |
| Number of reflections | 53028 | 4542 |
| <I/σ(I)> | 12 | 0.9 |
| Completeness [%] | 99.7 | 99.43 |
| Redundancy | 7 | 6.6 |
| CC(1/2) | 0.999 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298.15 | 16 - 17.8 % (w/v) PEG6000 or PEG3350, 0.1 M MES |
