9MS7

Crystal structure of a putative phage endolysin identified from a metagenomic survey of Prosser, Washington soil (PWe2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	92.046, 34.738, 110.760
Unit cell angles	90.00, 107.50, 90.00

Refinement procedure

Resolution	36.800 - 1.500
R-factor	0.1532
Rwork	0.151
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.739
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.800	1.530
High resolution limit [Å]	1.500	1.500
Rmeas	0.059
Rpim	0.022	0.460
Number of reflections	53615	2564
<I/σ(I)>	16.2	1.8
Completeness [%]	99.1
Redundancy	6.9
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M Magnesium Chloride, 0.1 M HEPES: NaOH, pH 7.5, 25 % (w/v) PEG 3350