9MS6

Crystal structure of a putative phage endolysin identified from a metagenomic survey of Prosser, Washington soil (PWe1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91966
Spacegroup name	P 32 2 1
Unit cell lengths	64.842, 64.842, 85.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.030 - 2.150
R-factor	0.2147
Rwork	0.212
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.030	2.220
High resolution limit [Å]	2.150	2.150
Rmeas	0.092
Rpim	0.015	0.531
Number of reflections	11764	971
<I/σ(I)>	27.9	1.4
Completeness [%]	99.9
Redundancy	39
CC(1/2)	1.000	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Hepes, sodium pH 7.5, 2% v/v PEG 400, 2.0 M ammonium sulfate