9MS0

Structure of cord Blood MAIT A2 TCR in complex with human MR1-5-OP-RU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	62.526, 114.533, 141.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 2.520
R-factor	0.1822
Rwork	0.180
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.840	2.590
High resolution limit [Å]	2.520	2.520
Number of reflections	34953	2871
<I/σ(I)>	10.2
Completeness [%]	99.6
Redundancy	2
CC(1/2)	0.997	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG3350, Na-acetate, BTP