9MRP
Crystal structures of a cyanobacterial DAP epimerase bound to L,L-aziDAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2024-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 179.460, 102.596, 47.674 |
| Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
| Resolution | 45.510 - 1.500 |
| R-factor | 0.2303 |
| Rwork | 0.230 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.510 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 264860 | 26460 |
| <I/σ(I)> | 7.53 | |
| Completeness [%] | 98.3 | 98.6 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.998 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291.15 | 0.1M Phosphate/citrate 4.2, 40% v/v PEG 300 |
