9MRH

Fluorescence lifetime-readout citrate sensor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2023-09-19
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	75.440, 96.190, 144.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.550 - 2.370
R-factor	0.222
Rwork	0.221
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.456
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.550	2.455
High resolution limit [Å]	2.370	2.370
Number of reflections	43193	4161
<I/σ(I)>	7.66
Completeness [%]	99.4
Redundancy	5.2
CC(1/2)	0.987	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Li2SO4, 0.1 M Bis-Tris pH 6.6, 16% PEG3350