9MRC
Crystal Structure of TREX1 Homolog Plex9.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.290, 96.930, 65.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.460 - 1.800 |
| R-factor | 0.1736 |
| Rwork | 0.172 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.121 | |
| Rmeas | 0.131 | |
| Rpim | 0.051 | |
| Number of reflections | 25307 | 4837 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.997 | 0.204 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 298 | 2.0M ammonium sulfate, 1.2M tri-sodium citrate pH |
