9MQW

Crystal structure of influenza virus N2 neuraminidase from A/Singapore/INFIMH-16-0019/2016 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	I 4 2 2
Unit cell lengths	137.320, 137.320, 155.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.550 - 2.290
R-factor	0.1768
Rwork	0.175
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.831
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.340
High resolution limit [Å]	2.290	2.290
Rpim	0.060	0.630
Number of reflections	32018	1258
<I/σ(I)>	12.6
Completeness [%]	95.4
Redundancy	17.6
CC(1/2)	0.993	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	0.1 M citric acid, pH 4.0, 2.4 M ammonium sulfate