9MQO

Crystal structure of BCMA in complex with CA10v2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	93.164, 94.784, 108.130
Unit cell angles	81.39, 81.40, 62.67

Refinement procedure

Resolution	83.847 - 3.181
Rwork	0.232
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.150	3.270
High resolution limit [Å]	3.180	3.180
Rmeas	0.235	0.595
Number of reflections	53814	3972
<I/σ(I)>	1.9	0.7
Completeness [%]	98.4	98.2
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.18 M Sodium Chloride, 0.09 M Phosphate/citrate pH 4.2, 20 % w/v PEG 8000, 0.1M Sodium Malonate