9MPX

Crystal structure of RM014, a macaque-derived HIV V2-apex-targeting antibody from ApexGT6 immunization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	98.809, 60.270, 81.995
Unit cell angles	90.00, 112.89, 90.00

Refinement procedure

Resolution	28.900 - 1.880
R-factor	0.1942
Rwork	0.191
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.861
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.880	1.880
Number of reflections	35396	1706
<I/σ(I)>	11.56
Completeness [%]	98.4
Redundancy	5.6
CC(1/2)	0.990	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Calcium acetate 0.1 M Tris pH 7.0 20% PEG 3000