9MPC

Crystal structure of the HIV V2-apex-targeting antibody RM018 from macaque

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	I 4 2 2
Unit cell lengths	118.702, 118.702, 194.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 3.300
R-factor	0.2547
Rwork	0.249
R-free	0.30730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.490
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	10810	519
<I/σ(I)>	7.6
Completeness [%]	100.0
Redundancy	15.5
CC(1/2)	0.997	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.16 M Zinc acetate 0.108 M Sodium cacodylate pH 6.5 14.4% PEG 8000, 20% Glycerol