9MLU
FbaA with Factor H 6-7 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-04-26 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.520, 79.077, 89.918 |
| Unit cell angles | 90.00, 91.83, 90.00 |
Refinement procedure
| Resolution | 36.190 - 1.820 |
| R-factor | 0.2285 |
| Rwork | 0.226 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.791 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.190 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 35778 | 35778 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.996 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MES pH 6.0, Sodium chloride, PEG 6000, Ethylene Glycol |
