9MK2
Crystal structure of Neisseria meningitidis ClpP protease complex with noncovalent activator, ACP1-01
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-17 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 97.350, 118.700, 127.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.270 - 1.950 |
| Rwork | 0.194 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.270 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.129 | 0.594 |
| Number of reflections | 107571 | 5228 |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.6 | 4.7 |
| CC(1/2) | 0.995 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.2 M potassium thiocyanate or potassium acetate, pH 7.5, 40% (+/-)-2-methyl-2,4-pentanediol |
