9MJV

Crystal structure of WRN helicase with bound allosteric fragment 2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-07-13
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	57.830, 58.364, 132.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.440 - 2.580
R-factor	0.23763
Rwork	0.235
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.379
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.440	2.620
High resolution limit [Å]	2.580	2.580
Number of reflections	14021	715
<I/σ(I)>	11.2
Completeness [%]	95.5
Redundancy	8.1
CC(1/2)	0.998	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	21-27.5% w/v PEG1500, 0.1 M MMT, pH 5.75-6.25