9MJT

Crystal structure of apo WRN helicase in Form D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-05-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	57.746, 58.208, 130.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.330 - 1.730
R-factor	0.21975
Rwork	0.217
R-free	0.25888
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.327
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.330	1.760
High resolution limit [Å]	1.730	1.730
Number of reflections	46791	2312
<I/σ(I)>	13.3	1.3
Completeness [%]	100.0
Redundancy	8.2	8.2
CC(1/2)	0.996	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	21-27.5% w/v PEG1500, 0.1 M MMT, pH 5.75-6.25