9MIW
Fatty Acid Binding Protein 4 (FABP4) Complexed with Perfluorooctanoic Acid (PFOA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-20 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.752, 37.716, 28.546 |
| Unit cell angles | 90.00, 93.02, 90.00 |
Refinement procedure
| Resolution | 22.510 - 1.240 |
| R-factor | 0.126 |
| Rwork | 0.124 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.520 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.028 | 0.125 |
| Rmeas | 0.029 | 0.174 |
| Rpim | 0.007 | 0.119 |
| Total number of observations | 519515 | 633 |
| Number of reflections | 36461 | 513 |
| <I/σ(I)> | 48.9 | 4.1 |
| Completeness [%] | 94.4 | |
| Redundancy | 14.2 | 1.2 |
| CC(1/2) | 1.000 | 0.942 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.55-1.6 M sodium citrate |
