9MIF

Crystal structure of the VRC01-class antibody 9C09, derived from GT1.1 vaccination, in complex with eOD-GT8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	53.005, 74.440, 208.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.230 - 1.930
R-factor	0.2354
Rwork	0.233
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.128
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.230	1.960
High resolution limit [Å]	1.930	1.930
Number of reflections	62686	2862
<I/σ(I)>	10.6
Completeness [%]	99.4
Redundancy	13.7
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	293	20% PEG-300, 10% glycerol, 5% (w/v) PEG-8000, 0.1M Tris pH 8.5, final pH 8.7