9MID
Crystal structure of the VRC01-class antibody 3G08, derived from GT1.1 vaccination, in complex with eOD-GT8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-30 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.105, 44.474, 103.977 |
| Unit cell angles | 90.00, 119.89, 90.00 |
Refinement procedure
| Resolution | 34.700 - 1.740 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.768 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 58145 | 2800 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium formate, 10% ethylene glycol, 20% polyethylene glycol 3350 |
